I-4   Dr. Dana Cialla, IPHT Jena

Innovative, metal nanoparticle-based technologies for bioanalytical application

Dr. Dana Cialla, Dr. Karina Weber, Prof. Dr. Jürgen Popp*

*Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany – www.ipc.uni-jena.de ↗
*Leibniz Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9, 07745 Jena, Germany – www.ipht-jena.de ↗

Modern analytical and diagnostic techniques should allow a fast, simple as well as robust, cost-efficient and automated detection. This contribution introduces innovative approaches for metal nanoparticle-based analytical methods to meet these requirements.

Based on electric-optical detection schemes, a biochip platform for DNA analysis is developed. Due to bimolecular interaction of two complementary molecules, DNA fragments of interest are bound on the biochip surface. Subsequently, an enzymatic generated silver deposition visualizes the binding event and an electric-optical readout is feasible. This chip-based detection method is demonstrated for the example of the detection of pathogens causing animal or plant diseases [1, 2]. Moreover, a detection scheme applying magnetic beads and the deposition of metallic nanoparticles to monitor the positive binding event is used for a DNA assay [3]. Finally, the optical properties of silver and gold nanostructures lead to a strong electromagnetic field enhancement due to the interaction with light, which is used for surface-enhanced Raman spectroscopy (SERS) [4, 5]. Thus, SERS combines the molecular fingerprint specificity of Raman spectroscopy with a high sensitivity. This readout technique is applied e.g. to monitor the enzyme activity [6] or to detect pathogens [7] within a microfluidic channel system.

Acknowledgement

Funding the projects "QuantiSERS" and "Jenaer Biochip Initiative 2.0" within the framework "InnoProfile Transfer – Unternehmen Region" the Federal Ministry of Education and Research, Germany (BMBF) is gratefully acknowledged. Furthermore, we thank for funding the project "PhytoValidierung" by the Federal Office for Agriculture and Food, Germany (BLE).

[1] B. Seise, A. Brinker, R. Kretschmer, M. Schwarz, B. Rudolph, T. Kaulfuß, M. Urban, T. Henkel, J. Popp, R. Möller, Engineering in Life Sciences, 11(2), 148-156 (2011).
[2] L. Schwenkbier, S. König, S. Wagner, S. Pollok, J. Weber, M. Hentschel, J. Popp, S. Werres, K. Weber, Microchimica Acta, published online, doi:10.1007/s00604-013-1107-3.
[3] M. Schwarz, S. Pahlow, T. Bocklitz, C. Steinbrücker, D. Cialla, K. Weber, J. Popp, Analyst, 138(20), 5866-5870 (2013).
[4] D. Cialla, A. März, R. Böhme, F. Theil, K. Weber, M. Schmitt, J. Popp, Analytical and Bioanalytical Chemistry, 403(1), 27-54 (2012).
[5] D. Cialla, S. Pollok, C. Steinbrücker, K. Weber, J. Popp, Nanophotonics, published online, doi:10.1515/nanoph-2013-0024
[6] A. März, B. Mönch, P. Rösch, M. Kiehntopf, T. Henkel, J. Popp, Analytical and Bioanalytical Chemistry, 400(9), 2755-2761 (2011).
[7] A. Walter, A. März, W. Schumacher, P. Rösch, J. Popp, Lab on a Chip, 11(12), 1013-1021 (2011).

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